Lord Chaos and Master Order exist together in the cosmos. Lord Chaos ask Master Order questions and Master Order attempts to answer them to Lord Chaos satisfaction. Master Order and Lord Chaos created the In-Betweener to maintain the balance of order and chaos within the universe. The In-Betweener planned to maintain a cosmic balance by creating universal insanity. He began by manipulating the Fantastic Four to fight amongst themselves over the Infinity Gems. Silver Surfer and Doctor Strange witnessed this and summoned Master Order and Lord Chaos who imprisoned the In-Betweener in the magic realm. When Thanos acquired the Infinity Gauntlet this got the attention of Master Order, Lord Chaos, Mistress Love, Master Hate, Galactus, Eternity, Kronos, and Adam Warlock. They joined forces and defeated Thanos when Adam Warlock stole his Infinity Gauntlet. The Living Tribunal interfered, not believing that Adam Warlock was worthy of the gauntlet. The Living Tribunal set up a trial for Adam Warlock which all those who defeated Thanos were there to oversee. Adam Warlock was ultimately deemed not worthy by The Living Tribunal and he was ordered to give up one of the Infinity Gems.
The Lord Chaos of Earth-616, as well as its alternate reality counterparts, was annihilated by the Beyonders.