Tracking Spider-Man to the apartment shared between Peter Parker and Officer Vin Gonzales, she mistakenly kidnapped Vin, believing him to be Spider-Man. Peter returned to the apartment unaware that Vin had been kidnapped, and believed Vin had found a Spider-Man costume in the apartment and figured out Peter's identity. In the sewers, Ana injected Vin with MGH to make him more of a challenge to hunt when Vermin found them both in his territory and attacked them. Unfortunately, Vermin was no match for Ana's superior hunting skills, and was ultimately defeated, leaving Ana to get back to her fun of hunting 'Spider-Man' in the sewers. While looking for Vin at his precinct, Peter disguised in one of Daredevil's costumes (which had eyelids to allow him to see) learned that a drug dealer had sold MGH to a young woman who claimed to have captured Spider-Man. Arriving at the dealer's territory, Peter was able to figure out where Vin was being held, running into and fighting Vermin along the way. Spider-Man (as Daredevil) battled Ana with Vin Gonzales and was able to rescue him when Vermin returned and attacked Ana. Although she failed in her hunt of Spider-Man, she brought Vermin home as a trophy for her mother.
Madame Web saw a vision of Ana hunting "spiders", and was eventually captured and held with Mattie Franklin by Ana and Sasha Kravinoff.